NKX2.1 Antibody / Lung Epithelial Cell Marker Antibody [clone SPM150] (V9087)

Description

NKX2.1 (NK2 homeobox 1) is a homeobox transcription factor that functions as a master regulator of pulmonary epithelial differentiation and respiratory epithelial lineage specification. NKX2.1 Antibody / Lung Epithelial Cell Marker Antibody (clone SPM150) recognizes this transcription factor, which is widely used as a marker of lung epithelial cell identity in studies of pulmonary tissue biology. NKX2.1 is also commonly referred to as Thyroid transcription factor 1 (TTF-1), and NKX2.1 antibody reagents are frequently used to identify epithelial cells of the respiratory epithelium.

During lung development and in the mature respiratory system, NKX2.1 acts as a lung lineage transcription factor controlling gene expression programs required for epithelial differentiation within the pulmonary epithelium. Expression of NKX2.1 is detected in epithelial cells lining the respiratory tract where it regulates transcriptional networks involved in pulmonary epithelial maturation, surfactant production, and maintenance of respiratory epithelial identity. Because NKX2.1 expression is tightly associated with lung epithelial lineage specification, NKX2.1 antibody reagents are widely used to study respiratory epithelium and pulmonary epithelial cell differentiation.

Within the distal lung, NKX2.1 expression is particularly enriched in alveolar type II cells, a specialized population of epithelial cells responsible for production and secretion of pulmonary surfactant. These cells play an essential role in maintaining alveolar stability and supporting normal respiratory function. Detection of NKX2.1 protein therefore provides a reliable approach for identifying alveolar epithelial cells and studying transcriptional programs governing pulmonary epithelial differentiation.

Because NKX2.1 expression is closely linked to pulmonary epithelial lineage identity, NKX2.1 antibody tools are widely used in research examining respiratory epithelium, lung epithelial cell biology, and alveolar epithelial differentiation. Detection of NKX2.1 protein allows investigators to identify epithelial cells of the respiratory tract and investigate molecular mechanisms controlling lung epithelial lineage specification. As a result, NKX2.1 antibody reagents serve as valuable tools for studying pulmonary epithelial differentiation and transcriptional regulation within respiratory epithelium.

Application Notes

The optimal dilution of the NKX2.1 Antibody / Lung Epithelial Cell Marker Antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length rat recombinant protein was used as the immunogen for this NKX2.1 Antibody / Lung Epithelial Cell Marker Antibody.

Storage

Store the NKX2.1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

TTF-1 antibody, Thyroid transcription factor 1 antibody, NKX2.1 transcription factor antibody, Pulmonary epithelial marker antibody