NKG2D Antibody / KLRK1 Antibody (RQ4042)

NKG2D Antibody EL-4 Cell FACS. Flow cytometry analysis of mouse EL-4 cells was performed using NKG2D Antibody / KLRK1 Antibody following fixation with 4% paraformaldehyde and blocking with normal goat serum. Red represents the unstained control, green the rabbit IgG isotype control, and blue the antibody-stained population. The pronounced rightward shift of the blue histogram relative to the isotype control demonstrates specific detection of endogenous KLRK1 expression in EL-4 cells. This NKG2D Antibody supports flow cytometric studies of KLRK1, natural killer cell activation, cytotoxic lymphocyte biology, and immune surveillance.

NKG2D Antibody Mouse EL-4 Cell and Rat Spleen WB. Western blot analysis of NKG2D Antibody / KLRK1 Antibody detected immunoreactive bands in rat spleen and mouse EL-4 cell lysates. Lanes: (1) rat spleen tissue and (2) mouse EL-4 whole cell lysates. The predominant band is observed at approximately 35 kDa, somewhat higher than the predicted molecular weight of 25 kDa, which is consistent with the extensive N-linked glycosylation known to occur on the extracellular domain of NKG2D. This post-translational modification commonly increases the apparent molecular weight observed by western blot. This NKG2D Antibody supports western blot studies of KLRK1, natural killer cell activation, cytotoxic lymphocyte biology, and immune surveillance.