NIT1 Antibody / Deaminated glutathione amidase Antibody (FY13620)

NIT1 Antibody by IHC in human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate carcinoma using Deaminated glutathione amidase Antibody shows prominent brown cytoplasmic staining in glandular epithelial cells. Antigen retrieval was performed with EDTA buffer at pH 8.0. Sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC. Detection used an HRP-conjugated secondary antibody and DAB chromogen, with hematoxylin counterstaining.

NIT1 Antibody by WB in human cell lines. Western blot analysis using Deaminated glutathione amidase Antibody detects an immunoreactive band consistent with the expected molecular weight of approximately 36 kDa. Lane assignments: HepG2 (lane 1), HeLa (lane 2). Each lane contained 30 ug total protein under reducing conditions, resolved by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. An HRP-conjugated secondary antibody and chemiluminescent substrate were used for detection.

NIT1 Antibody by IHC in human thyroid carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human thyroid carcinoma using Deaminated glutathione amidase Antibody shows brown staining in epithelial cells surrounding glandular structures. Antigen retrieval was performed with EDTA buffer at pH 8.0. Sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC. Detection used an HRP-conjugated secondary antibody and DAB chromogen, with hematoxylin counterstaining.