NGFR Antibody NGFR/4919 / p75 Neurotrophin Receptor Antibody [clone NGFR/4919] (V9706)

NGFR Antibody NGFR/4919 by IHC in Human Melanoma. Immunohistochemistry staining of FFPE human melanoma tissue using mouse monoclonal NGFR antibody NGFR/4919 at 2 ug/ml. Strong brown immunoreactivity is observed in irregular clusters and branching groups of tumor cells, with predominantly membranous and peripheral cytoplasmic staining. Surrounding tissue exhibits comparatively weak staining, highlighting the distribution of p75 neurotrophin receptor (CD271) expression. The negative control inset shows minimal background staining when PBS replaces the primary antibody. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling.

NGFR Antibody NGFR/4919 by IHC Showing Heterogeneous Staining in Human Melanoma. Immunohistochemistry staining of FFPE human melanoma tissue using mouse monoclonal NGFR antibody NGFR/4919 at 2 ug/ml. Prominent brown immunoreactivity is concentrated in discrete groups of tumor cells, with staining outlining cellular boundaries and extending into peripheral cytoplasmic regions. The surrounding tissue demonstrates relatively weak staining, producing a clear contrast between NGFR-positive and less reactive areas. This distribution illustrates heterogeneous expression of p75 neurotrophin receptor (CD271) within melanoma tissue. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling.

NGFR Antibody NGFR/4919 by SDS-PAGE Under Reducing and Non-Reducing Conditions. SDS-PAGE analysis of purified, BSA-free mouse monoclonal NGFR antibody NGFR/4919, with 2 ug of antibody loaded per lane. Under non-reducing (NR) conditions, the intact antibody migrates predominantly near 150 kDa. Under reducing (R) conditions, heavy and light chains are observed at approximately 50 kDa and 25 kDa, respectively. These results support the structural integrity of the antibody used to detect p75 neurotrophin receptor, also known as CD271 and TNFRSF16.

NGFR Antibody NGFR/4919 by HuProt Protein Microarray. Protein microarray analysis of mouse monoclonal NGFR antibody NGFR/4919 using a HuProt array containing more than 19,000 full-length human proteins. NGFR produces the dominant binding signal, with substantially lower signals observed for other proteins represented on the array. The Z-score measures signal intensity relative to the overall array distribution, while the S-score reflects separation between the highest-ranking targets. These results provide additional evidence of preferential recognition of NGFR, also known as p75 neurotrophin receptor (CD271), by clone NGFR/4919.