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Home >> Antibodies >> NGFR Antibody

NGFR Antibody [clone 8G3G10] (F53510)

  Catalog No Formulation Size Price (USD)  
Image F53510-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F53510-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Fluorescent confocal image of SY5Y cells stained with NGFR antibody. Alexa Fluor 488 secondary Ab (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
Western blot analysis of NGFR antibody and mouse heart tissue lysate. Predicted molecular weight ~45/75kDa (unmodified/glycosylated).
NGFR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, k
Clone Name 8G3G10
Purity Purified
UniProt P08138
Applications Immunofluorescence : 1:100
Western blot : 1:500-1:1000
IHC (Paraffin) : 1:50-1:100
Limitations This NGFR antibody is available for research use only.
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Description

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

Application Notes

Titration of the NGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This NGFR antibody was produced from mouse immunized with NGFR recombinant protein.

Storage

Aliquot the NGFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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