NGF Receptor Polyclonal Antibody / p75 NGF Receptor Antibody (RQ5997)

NGF Receptor Polyclonal Antibody by IF in Human A431 Cells. Immunofluorescence staining of FFPE human A431 cells using rabbit polyclonal NGF Receptor antibody. Green fluorescence is visible predominantly along the cell periphery, with additional cytoplasmic staining surrounding the blue DAPI-stained nuclei. The staining pattern is consistent with the cellular distribution of p75 NGF Receptor (CD271), a transmembrane protein involved in neurotrophin signaling. Heat-induced epitope retrieval was performed by steaming sections in citrate buffer, pH 6, for 20 minutes.

NGF Receptor Polyclonal Antibody by WB in Rat and Human Lysates. Western blot analysis of rat brain (lane 1), human SW620 cells (lane 2), and human Caco-2 cells (lane 3) using rabbit polyclonal NGF Receptor antibody. A prominent immunoreactive band is observed near 45-50 kDa in all three lanes, with additional weaker bands near 70-75 kDa in the human samples. The lower molecular weight band is consistent with the predicted unmodified p75 NGF Receptor, while the higher molecular weight signals may represent glycosylated forms of NGFR (CD271). The results demonstrate antibody reactivity in both rat and human lysates, with comparable migration of the predominant bands.

NGF Receptor Polyclonal Antibody by FACS in Human A431 Cells. Flow cytometry analysis of human A431 cells using rabbit polyclonal NGF Receptor antibody at 1 ug per million cells following blocking with goat serum. The antibody-stained population (blue) exhibits a clear rightward fluorescence shift relative to the isotype control (green) and unstained cells (red). The separation between the antibody-stained and control populations demonstrates detectable p75 NGF Receptor (CD271) expression in A431 cells. The histogram provides evidence of antibody performance in flow cytometry and supports its use in studies of NGFR-associated cell surface expression.