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Home >> Antibodies >> NFRKB Antibody / Nuclear factor related to kappa-B-binding protein

NFRKB Antibody / Nuclear factor related to kappa-B-binding protein (RQ8132)

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  Catalog No Formulation Size Price (USD)  
Image RQ8132 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A549 cells with NFRKB antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with NFRKB antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEL, 2) RT-4, 3) Caco-2 and 4) HaCaT cell lysate with NFRKB antibody. Predicted molecular weight ~139 kDa, commonly observed at ~175 kDa and can be observed at ~220 kDa.
Flow cytometry testing of fixed and permeabilized human HEL cells with NFRKB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NFRKB antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q6P4R8
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NFRKB antibody is available for research use only.
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Description

NFRKB (nuclear factor related to kappa-B-binding protein) was first identified as a factor that could bind a variant NF-kappa B site in the interleukin-2 receptor alpha (IL-2R alpha) gene. NFRKB appears to be a transcription factor central to immune cell development. Evidence of this comes from the identification of NFRKB as an amplified gene in several cases of acute myeloid leukemia. Additionally, in another clinical case, a partial deletion of the chromosomal region that includes NFRKB is shown to be associated with combined cellular immunodeficiency.

Application Notes

Optimal dilution of the NFRKB antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids H270-L994) was used as the immunogen for the NFRKB antibody.

Storage

After reconstitution, the NFRKB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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