NF-M Antibody / Neurofilament Medium Chain Antibody [clone AAGI-14] (RQ5169)

NF-M Antibody Human 293T Cell WB. Western blot testing of human 293T cell lysate using NF-M Antibody, clone AAGI-14. A prominent band is detected at approximately 145-160 kDa, consistent with the characteristic migration of heavily phosphorylated neurofilament medium chain, despite a predicted molecular weight of approximately 102 kDa. The slower electrophoretic mobility reflects extensive post-translational phosphorylation, a well-established feature of NF-M. These results demonstrate that NF-M Antibody, a Neurofilament Medium Chain Antibody, specifically detects endogenous NF-M in human cells.

NF-M Antibody Human 293T and SH-SY5Y WB. Western blot analysis of human 293T and SH-SY5Y cell lysates using NF-M Antibody. Lane 1: 293T whole cell lysate. Lane 2: SH-SY5Y whole cell lysate. Samples (30 ug/lane) were separated on an 8% SDS-PAGE gel under reducing conditions, transferred to a nitrocellulose membrane, and detected with NF-M Antibody followed by an HRP-conjugated secondary antibody and ECL detection. A strong band is observed at approximately 160 kDa, migrating above the predicted molecular weight of approximately 102 kDa due to the extensive phosphorylation characteristic of neurofilament medium chain. These results demonstrate that NF-M Antibody, a Neurofilament Medium Chain Antibody, specifically detects endogenous NEFM in human cells.

NF-M Antibody Rat Cerebellum IHC. Immunohistochemical analysis of FFPE rat cerebellum tissue using NF-M Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Distinct cytoplasmic and neurite staining is observed in cerebellar neurons, with prominent immunoreactivity within the Purkinje cell layer and neuronal processes, consistent with the expected distribution of neurofilament medium chain. These results demonstrate that NF-M Antibody, a Neurofilament Medium Chain Antibody, specifically detects endogenous NEFM in FFPE rat nervous tissue.

NF-M Antibody Pig Brain IHC. Immunohistochemical analysis of FFPE pig brain tissue using NF-M Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate cytoplasmic staining is observed in neurons throughout the brain tissue, consistent with the expected expression pattern of neurofilament medium chain within the neuronal cytoskeleton. These results demonstrate that NF-M Antibody, a Neurofilament Medium Chain Antibody, specifically detects endogenous NEFM in FFPE porcine nervous tissue.