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Home >> Antibodies >> NF-H Antibody (phospho)

NF-H Antibody (phospho) [clone NE14] (V2759)

  Catalog No Formulation Size Price (USD)  
Image V2759-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V2759-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V2759SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
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V2759IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 499
IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with NF-H antibody (NE14).
IHC: Formalin-fixed, paraffin-embedded rat cerebellum stained with NF-H antibody (NE14).
SDS-PAGE Analysis of Purified, BSA-Free NF-H Antibody (clone NE14). Confirmation of Integrity and Purity of the Antibody.
Western blot testing of human brain lysate with NF-H antibody (clone NE14).
Flow cytometry testing of permeabilized human HEK293 cells with NF-H antibody (clone NE14); Red=isotype control, Blue= NF-H antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name NE14
Purity Protein G affinity chromatography
UniProt P12036
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Flow cytometry : 0.5-1ug/10^6 cells
Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations This NF-H antibody (phospho) is available for research use only.
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Description

This mAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

Application Notes

Optimal dilution of the NF-H antibody (phospho) should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho).

Storage

Store the NF-H antibody (phospho) at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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