- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
NF-H Antibody / Neurofilament (Heavy) / NEFH (Center Region) (F54187)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
F54187-0.2ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.2 ml | 439 | |
|
|
F54187-0.05ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.05 ml | 205 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity |
| UniProt | P12036 |
| Applications | Western Blot : 1:1000-1:2000 |
| Limitations | This NF-H antibody is available for research use only. |
|
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Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
For additional research on NF-H expression, axonal structure and neurofilament biology, see our Neurofilament Heavy Chain Antibody / Axonal Cytoskeleton Marker page.
The stated application concentrations are suggested starting points. Titration of the NF-H antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 328-362 from human NEFH was used as the immunogen for the NF-H antibody.
Aliquot the NF-H antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Citations(8)
![Western blot testing of human prostate lysate with GPX7 antibody at 0.3ug/ml with [B] and without [A] blocking/immunizing peptide. The expected ~20 kDa band and the additional ~40 kDa band are both blocked by the immunizing peptide.](https://www.nsjbio.com/plp/thumb_image/img-R33292-100UG-1.jpg?v=1788318968 "Western blot testing of human prostate lysate with GPX7 antibody at 0.3ug/ml with [B] and without [A] blocking/immunizing peptide. The expected ~20 kDa band and the additional ~40 kDa band are both blocked by the immunizing peptide.")
![Western blot testing of human [A], mouse [B] and rat [C] lung lysates with DLL4 antibody at 0.3ug/ml. Predicted/observed molecular weight 75~85kDa.](https://www.nsjbio.com/plp/thumb_image/img-R34555-100UG-1.jpg?v=1788318968 "Western blot testing of human [A], mouse [B] and rat [C] lung lysates with DLL4 antibody at 0.3ug/ml. Predicted/observed molecular weight 75~85kDa.")
![HEK293 lysate overexpressing human HOXA1-MYC probed with HOXA1 [1ug/ml] in Lane A and anti-MYC tag [1/1000] in lane C. Mock-transfected HEK293 probed with HOXA1 antibody [1ug/ml] in Lane B. Predicted molecular weight: ~37/15kDa (isoforms a/b).](https://www.nsjbio.com/plp/thumb_image/img-R35385-100UG-1.jpg?v=1788318968 "HEK293 lysate overexpressing human HOXA1-MYC probed with HOXA1 [1ug/ml] in Lane A and anti-MYC tag [1/1000] in lane C. Mock-transfected HEK293 probed with HOXA1 antibody [1ug/ml] in Lane B. Predicted molecular weight: ~37/15kDa (isoforms a/b).")
