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Home >> Antibodies >> Neuropilin 1 Antibody / NRP1

Neuropilin 1 Antibody / NRP1 [clone 4G3F7] (RQ7016)

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  Catalog No Formulation Size Price (USD)  
Image RQ7016 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A549 cells with Neuropilin 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with Neuropilin 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human PC-3, 2) human U-87 MG, 3) rat brain and 4) rat C6 cell lysate with Neuropilin 1 antibody. Expected molecular weight: 102-130 kDa.
Flow cytometry testing of human U-87 MG cells with Neuropilin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Neuropilin 1 antibody.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 4G3F7
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O14786
Localization Cytoplasmic
Applications Western blot : 0.5-1 ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Neuropilin 1 antibody is available for research use only.
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Description

This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contain a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

Application Notes

Optimal dilution of the Neuropilin 1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids K504-T827) was used as the immunogen for the Neuropilin 1 antibody.

Storage

After reconstitution, the Neuropilin 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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