Neurofilament Light Antibody / Neuronal Intermediate Filament Antibody (RQ6261)

Neurofilament Light Antibody Mouse Spinal Cord IHC. Immunohistochemical staining of FFPE mouse spinal cord tissue with Neurofilament Light antibody. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool prior to staining. Neurofilament Light immunoreactivity is observed within neuronal cell bodies and extensive axonal processes, consistent with the expected localization of this neuronal intermediate filament protein that provides structural support for axons and helps maintain neuronal architecture. Cell nuclei are counterstained with hematoxylin (blue).

Neurofilament Light Antibody Rat Spinal Cord IHC. Immunohistochemical staining of FFPE rat spinal cord tissue with Neurofilament Light antibody. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool prior to staining. Neurofilament Light immunoreactivity is observed within neuronal cell bodies and axonal processes, consistent with the expected localization of this neuronal intermediate filament protein that provides structural support for axons and helps maintain neuronal architecture. Cell nuclei are counterstained with hematoxylin (blue).

Neurofilament Light Antibody Multi-Species Brain WB. Western blot testing of 1) human SH-SY5Y, 2) rat brain, and 3) mouse brain lysates with Neurofilament Light antibody. A prominent immunoreactive band is detected at approximately 68 kDa in all three samples, consistent with the predicted molecular weight range of Neurofilament Light (62-68 kDa). The results demonstrate specific detection of this neuronal intermediate filament protein, which is abundantly expressed in neurons and plays an essential role in maintaining axonal structure and neuronal integrity.

Flow cytometry testing of human 293T cells with Neurofilament Light antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Neurofilament Light antibody.

Neurofilament Light Antibody Mouse Spinal Cord IHC with Negative Control. Immunohistochemical staining of FFPE mouse spinal cord tissue with Neurofilament Light antibody (right). Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool prior to staining. Neurofilament Light immunoreactivity is observed within neuronal cell bodies and extensive axonal processes, consistent with the expected localization of this neuronal intermediate filament protein that provides structural support for axons and helps maintain neuronal architecture. The negative control (left), in which the primary antibody was omitted, showed no specific staining. Cell nuclei are counterstained with hematoxylin (blue).

Neurofilament Light Antibody Mouse Brain IF. Immunofluorescent staining of FFPE mouse brain tissue with Neurofilament Light antibody (red). Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool prior to staining. Nuclei are counterstained with DAPI (blue). Neurofilament Light immunoreactivity is observed throughout neuronal cell bodies and extensive neurites, consistent with the expected localization of this neuronal intermediate filament protein that provides structural support for axons and helps maintain neuronal architecture.

Neurofilament Light Antibody Rat Brain IF. Immunofluorescent staining of FFPE rat brain tissue with Neurofilament Light antibody (red). Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool prior to staining. Nuclei are counterstained with DAPI (blue). Neurofilament Light immunoreactivity is observed prominently within neuronal processes throughout the brain parenchyma, consistent with the expected localization of this neuronal intermediate filament protein that provides structural support for axons and helps maintain neuronal architecture.

Neurofilament Light Antibody Human Brain IHC. Immunohistochemical staining of FFPE human brain tissue with Neurofilament Light antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Neurofilament Light immunoreactivity is observed within neuronal cell bodies and extensive axonal processes, consistent with the expected localization of this neuronal intermediate filament protein that provides structural support for axons and helps maintain neuronal architecture. Cell nuclei are counterstained with hematoxylin (blue).

Neurofilament Light Antibody Human Hippocampus IHC. Immunohistochemical staining of FFPE human hippocampus tissue with Neurofilament Light antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Neurofilament Light immunoreactivity is observed within neuronal cell bodies and extensive axonal processes throughout the hippocampus, consistent with the expected localization of this neuronal intermediate filament protein that provides structural support for axons and helps maintain neuronal architecture. Cell nuclei are counterstained with hematoxylin (blue).