• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Neurofilament Heavy Antibody

Neurofilament Heavy Antibody [clone RT97] (V2213)

  Catalog No Formulation Size Price (USD)  
Image V2213-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
Image
V2213-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
Image
V2213SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
Image
V2213IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of cerebellum stained with Neurofilament Heavy antibody (RT97).
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name RT97
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 4744
Localization Cytoplasmic, membranous
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations This Neurofilament Heavy antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

This antibody reacts with a 200kDa protein, identified as heavy subunit of neurofilaments, or NF-H. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H), plus alpha internexin or peripherin. Each neurofilament subunit contains a globular head, an alpha helical rod domain, and variable tail domains that differ in length and amino acid content.

Neurofilament antibody stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for neurofilament antibody. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

The triton-X 100 insoluble protein fraction of rat brain was used as the immunogen for this Neurofilament Heavy antibody.

Storage

Store the Neurofilament Heavy antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

NEFH; Neurofilament H; Neurofilament Heavy Polypeptide 200kDa; NF-H; NF200, Neurofilament heavy antibody

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.