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Home >> Antibodies >> Neurofilament heavy Antibody / Nefh

Neurofilament heavy Antibody / Nefh (RQ6260)

  Catalog No Formulation Size Price (USD)  
Image RQ6260 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE mouse spinal cord with Neurofilament heavy antibody (right) and negative control (left). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with Neurofilament heavy antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain and 2) mouse brain lysate with Neurofilament heavy antibody. Predicted molecular weight ~112 kDa, often visualized at ~200 kDa.
Flow cytometry testing of mouse Neuro-2a cells with Neurofilament heavy antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Neurofilament heavy antibody.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P19246
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Neurofilament heavy antibody is available for research use only.
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Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Application Notes

Optimal dilution of the Neurofilament heavy antibody should be determined by the researcher.

Immunogen

A mouse recombinant partial protein (amino acids Y109-E466) was used as the immunogen for the Neurofilament heavy antibody.

Storage

After reconstitution, the Neurofilament heavy antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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