Neurofilament Heavy Antibody / Neuronal Intermediate Filament Antibody (RQ6260)

Neurofilament Heavy Antibody Human Cerebellum IHC. Immunohistochemical staining of FFPE human cerebellum tissue with Neurofilament Heavy antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Neurofilament Heavy immunoreactivity is observed predominantly within neuronal cell bodies and extensive axonal processes throughout the cerebellum, with particularly strong staining of the cerebellar white matter, consistent with the expected localization of this neuronal intermediate filament protein that provides structural support for axons and maintains neuronal architecture. Cell nuclei are counterstained with hematoxylin (blue).

Neurofilament Heavy Antibody Rat Brain IHC. Immunohistochemical staining of FFPE rat brain tissue with Neurofilament Heavy antibody. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool prior to staining. Neurofilament Heavy immunoreactivity is observed predominantly within neuronal cell bodies and axonal processes, consistent with the expected localization of this neuronal intermediate filament protein that provides structural support for axons and helps maintain neuronal architecture. Cell nuclei are counterstained with hematoxylin (blue).

Neurofilament Heavy Antibody Rodent Brain WB. Western blot testing of 1) rat brain and 2) mouse brain lysates with Neurofilament Heavy antibody. A strong immunoreactive band is detected at approximately 200 kDa in both brain lysates, consistent with the highly phosphorylated form of Neurofilament Heavy that commonly migrates well above its predicted molecular weight of approximately 112 kDa. The results demonstrate specific detection of this neuronal intermediate filament protein in both rat and mouse brain tissue.

Flow cytometry testing of mouse Neuro-2a cells with Neurofilament heavy antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Neurofilament heavy antibody.

Neurofilament Heavy Antibody Mouse Spinal Cord IHC. Immunohistochemical staining of FFPE mouse spinal cord tissue with Neurofilament Heavy antibody (right). Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool prior to staining. Neurofilament Heavy immunoreactivity is observed within neuronal cell bodies and axonal processes, consistent with the expected localization of this neuronal intermediate filament protein that provides structural support for axons and maintains neuronal architecture. The negative control (left), in which the primary antibody was omitted, showed no specific staining. Cell nuclei are counterstained with hematoxylin (blue).