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Home >> Antibodies >> Neurofilament Antibody / Heavy

Neurofilament Antibody / Heavy [clone NF421] (V2212)

  Catalog No Formulation Size Price (USD)  
Image V2212-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2212-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2212SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2212IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of FFPE human cerebellum with Neurofilament antibody (clone NF421).
SDS-PAGE Analysis of Purified, BSA-Free Neurofilament Antibody (clone NF421). Confirmation of Integrity and Purity of the Antibody.
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name NF421
Purity Protein G purified Neurofilament antibody
Buffer 1X PBS, pH 7.4
Gene ID 4744
Localization Cytoplasmic, membranous
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations This Neurofilament antibody is available for research use only.
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Description

Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.

Application Notes

The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.

1. The NF421 antibody is specific to the heavy subunit.
2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human recombinant Neurofilament protein was used as the immunogen. (1)

Storage

Store the Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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