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Home >> Antibodies >> Neurofilament 200 Antibody (Heavy)

Neurofilament 200 Antibody (Heavy) [clone N52, aka NF-200] (R30062)

  Catalog No Formulation Size Price (USD)  
Image R30062 0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS 100 ug 329
IHC-P: Neurofilament 200 antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Ascites
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name N52, aka NF-200
Purity Ascites
Gene ID 4744
Localization Cytoplasmic, membranous
Applications Western blot : 0.5ml
IHC (FFPE) : 1-2ug/ml
IHC (Frozen) : 1-2ug/ml; formalin or acetone fixed tissues
Limitations This Neurofilament 200 antibody is available for research use only.
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Description

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68kD (NFL), 125kD (NFM), and 200kD (NFH) on SDS-gel electrophoresis. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Genomic clones for the largest human neurofilament protein were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. Mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease(CMT).

Application Notes

Titration of the Neurofilament 200 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200 was used as the immunogen for this Neurofilament 200 antibody.

Storage

Aliquot and store the Neurofilament 200 antibody at -20oC. Avoid repeated freezing and thawing.

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