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Home >> Antibodies >> Neural Cell Adhesion Molecule 1 Antibody

Neural Cell Adhesion Molecule 1 Antibody (F54748)

  Catalog No Formulation Size Price (USD)  
Image F54748-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F54748-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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Neural Cell Adhesion Molecule 1 Antibody Human Cerebellum IHC. Immunohistochemical staining of FFPE human cerebellum tissue with Neural Cell Adhesion Molecule 1 Antibody demonstrates NCAM1/CD56 expression. Heat-induced epitope retrieval was performed by steaming sections in pH9 EDTA buffer for 20 minutes followed by cooling. Brown staining is observed throughout the cerebellar tissue, with hematoxylin counterstaining in blue. This Neural Cell Adhesion Molecule 1 Antibody, also called NCAM1 Antibody, supports studies of neural cell adhesion, neuronal development, and cerebellar biology.
Neural Cell Adhesion Molecule 1 Antibody Human Brain IHC. Immunohistochemical staining of FFPE human brain tissue with Neural Cell Adhesion Molecule 1 Antibody demonstrates NCAM1/CD56 expression. Heat-induced epitope retrieval was performed by steaming sections in pH9 EDTA buffer for 20 minutes followed by cooling. Strong brown staining is observed throughout the neural tissue, with hematoxylin counterstaining in blue. This Neural Cell Adhesion Molecule 1 Antibody, also called NCAM1 Antibody, supports studies of neural cell adhesion, neuronal development, and nervous system biology.
Neural Cell Adhesion Molecule 1 Antibody Human, Mouse and Rat Brain WB. Western blot testing of human brain (lane 1), mouse brain (lane 2), and rat brain (lane 3) tissue lysates demonstrates detection of multiple NCAM1 isoforms. Neural Cell Adhesion Molecule 1 has predicted molecular weights of approximately 110 kDa for the soluble fragment, 120-125 kDa for GPI-anchored forms, and 140-180 kDa for transmembrane isoforms. Higher apparent molecular weights may also reflect extensive post-translational modification. This Neural Cell Adhesion Molecule 1 Antibody, also called NCAM1 Antibody, supports studies of NCAM1 isoform expression, neural cell adhesion, and nervous system biology.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
UniProt P13591
Localization Cell surface, cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:25
Western Blot : 1:500-1:2000
Limitations This Neural Cell Adhesion Molecule 1 Antibody is available for research use only.
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Description

Neural Cell Adhesion Molecule 1 Antibody recognizes NCAM1, also known as CD56, a cell-surface glycoprotein belonging to the immunoglobulin superfamily. Neural Cell Adhesion Molecule 1 mediates cell-cell interactions and participates in adhesion, migration, differentiation, and intracellular signaling. NCAM1 is particularly abundant in neural tissues, where it contributes to nervous system development, neurite extension, axonal guidance, synaptic organization, and neural plasticity. Multiple NCAM1 isoforms are produced through alternative splicing, including membrane-associated and soluble forms with distinct structural and functional properties.

NCAM1 undergoes extensive post-translational modification that influences its molecular characteristics and biological activity. Polysialylation is particularly important because addition of polysialic acid can reduce NCAM1-mediated adhesion and facilitate dynamic cellular interactions during development, migration, and tissue remodeling. NCAM1 also interacts with cell-surface receptors, extracellular matrix components, and intracellular signaling proteins, allowing it to influence cytoskeletal organization, cellular motility, survival, and differentiation. A Neural Cell Adhesion Molecule 1 Antibody can support research into NCAM1 expression, localization, and its roles in neural development and cell adhesion.

Outside the nervous system, NCAM1 is widely recognized as the CD56 antigen and is prominently expressed by natural killer cells as well as subsets of T lymphocytes. CD56 is consequently an important marker in immunology and immune cell characterization. NCAM1 expression is also associated with several malignancies, particularly tumors exhibiting neural, neuroendocrine, or hematologic differentiation. Altered expression may influence tumor cell adhesion, migration, signaling, and interactions with surrounding cells. Neural Cell Adhesion Molecule 1 Antibody reagents are therefore relevant to research spanning neuroscience, immunology, developmental biology, and cancer biology.

The diverse functions of NCAM1 connect cell adhesion with signaling mechanisms involved in tissue development, neural function, immunity, and disease. Its expression as CD56 further provides an important link between neural cell adhesion biology and immune cell phenotyping. NSJ Bioreagents offers antibodies for studying CD markers and related cellular proteins. A Neural Cell Adhesion Molecule 1 Antibody is useful for studying NCAM1 expression, CD56 biology, neuronal development, cell-cell adhesion, immune cell populations, and NCAM1-associated cancers.

For additional information on NCAM1/CD56 and its roles in neural cell adhesion, neuronal development, and cell signaling, see our NCAM1 Antibody / Neural Cell Adhesion Molecule 1 Antibody page.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Neural Cell Adhesion Molecule 1 Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 796-830 from the human protein was used as the immunogen for the Neural Cell Adhesion Molecule 1 Antibody.

Storage

Aliquot the Neural Cell Adhesion Molecule 1 Antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

Neural Cell Adhesion Molecule 1 antibody, NCAM1 antibody, CD56 antibody, NCAM antibody, N-CAM antibody, CD56 antigen antibody

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