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Home >> Antibodies >> NeuN Antibody / Fox3

NeuN Antibody / Fox3 [clone AO-18] (RQ4501)

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Image RQ4501 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Immunofluorescent staining of human SH-SY5Y cells with NeuN antibody (green) and DAPI nuclear stain (blue).
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Immunofluorescent staining of human SH-SY5Y cells with NeuN antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human pancreas with NeuN antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human fetal brain lysate with NeuN antibody at 0.5ug/ml. Expected molecular weight: 46-48 kDa (two isoforms may be visualized).
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name AO-18
Purity Protein A affinity
UniProt A6NFN3
Localization Nucleus, Cytoplasm
Applications Western blot : 1:1000-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence : 1:50-1:200
Limitations This NeuN antibody is available for research use only.
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Description

RNA binding protein fox-1 homolog 3 is a RNA-binding protein that regulates alternative splicing events. [UniProt]

Application Notes

Optimal dilution of the NeuN antibody should be determined by the researcher.

Immunogen

A synthetic peptide from human NeuN was used as the immunogen for the NeuN antibody.

Storage

After reconstitution, the NeuN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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