NeuN Antibody / RBFOX3 Neuronal Nuclear Marker [clone AO-18] (RQ4501)

NeuN Antibody Neuronal Nuclear IF. Immunofluorescence analysis of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in human SH-SY5Y cells using recombinant rabbit monoclonal NeuN antibody, clone AO-18. Green fluorescence highlights strong nuclear localization in neuronal cells, consistent with NeuN as a marker of mature neurons, while nuclei are counterstained with DAPI (blue).

NeuN Antibody Pancreatic Tissue IHC. Immunohistochemistry of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in FFPE human pancreas using recombinant rabbit monoclonal NeuN antibody, clone AO-18, at 2 ug/ml. HRP-DAB staining shows minimal to absent nuclear labeling in pancreatic epithelial cells, consistent with the neuron-restricted expression of NeuN, while nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 6 10 mM citrate buffer for 10-20 min followed by cooling at RT for 20 min.

NeuN Antibody Fetal Brain WB. Western blot analysis of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in human fetal brain lysate using recombinant rabbit monoclonal NeuN antibody, clone AO-18, at 0.5 ug/ml. Two bands are detected at approximately 46-48 kDa, consistent with the expected molecular weight of RBFOX3 isoforms, supporting detection of this neuronal nuclear protein in developing neural tissue.

NeuN Antibody Brain Tissue WB. Western blot analysis of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in rat brain (Lane 1) and mouse brain (Lane 2) tissue lysates using recombinant rabbit monoclonal NeuN antibody, clone AO-18. Two bands are detected at approximately 46-48 kDa in both lanes, consistent with the expected molecular weight of RBFOX3 isoforms, supporting conserved expression of this neuronal nuclear protein across species.

NeuN Antibody Mouse Brain IHC. Immunohistochemistry of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in FFPE mouse brain tissue using recombinant rabbit monoclonal NeuN antibody, clone AO-18. HRP-DAB brown staining highlights strong nuclear labeling of neurons in distinct cortical and hippocampal layers, consistent with NeuN as a marker of mature neuronal populations, while surrounding glial cells show minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

NeuN Antibody Cerebellar Layer IHC. Immunohistochemistry of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in FFPE mouse cerebellum tissue using recombinant rabbit monoclonal NeuN antibody, clone AO-18. HRP-DAB brown staining highlights strong nuclear labeling of neurons within the granular layer, with minimal staining in the molecular layer, consistent with NeuN expression in mature neuronal populations, while nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

NeuN Antibody Rat Brain IHC. Immunohistochemistry of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in FFPE rat brain tissue using recombinant rabbit monoclonal NeuN antibody, clone AO-18. HRP-DAB brown staining highlights widespread nuclear labeling of neurons throughout the brain parenchyma, consistent with NeuN as a marker of mature neuronal populations, while non-neuronal cells show minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.

NeuN Antibody Rat Cerebellar IHC. Immunohistochemistry of RNA binding fox-1 homolog 3 / RBFOX3 (NeuN) in FFPE rat cerebellum tissue using recombinant rabbit monoclonal NeuN antibody, clone AO-18. HRP-DAB brown staining highlights dense nuclear labeling of neurons within the granular layer, with minimal staining in the molecular layer, consistent with NeuN expression in mature cerebellar neuronal populations, while nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining.