NEK7 Antibody / Inflammasome Activation Protein Antibody (FY12050)

NEK7 Antibody SiHa Cell IF. Immunofluorescent staining of human SiHa cells with NEK7 Antibody (green) and beta Tubulin antibody (red). Enzyme antigen retrieval was performed for 15 minutes, followed by blocking with 10% goat serum. Cells were incubated with rabbit anti-NEK7 antibody at 5 ug/ml and mouse anti-beta Tubulin antibody overnight at 4°C, followed by DyLight 488 and Cy3 conjugated secondary antibodies. NEK7 Antibody / Inflammasome Activation Protein Antibody demonstrates staining consistent with NIMA Related Kinase 7 expression and supports studies of NLRP3 inflammasome regulation, innate immune signaling and cell cycle pathways.

NEK7 Antibody Human, Rat and Mouse WB. Western blot testing of human 293T, HeLa, Jurkat and SiHa cell lysates, along with rat heart, mouse lung and mouse heart tissue lysates, with NEK7 Antibody at 0.5 ug/ml. Lane 1: human 293T; Lane 2: human HeLa; Lane 3: human Jurkat; Lane 4: human SiHa; Lane 5: rat heart; Lane 6: mouse lung; Lane 7: mouse heart. A specific band was detected at approximately 32 kDa. NEK7 Antibody / Inflammasome Activation Protein Antibody detects endogenous NIMA Related Kinase 7 expression and supports studies of NLRP3 inflammasome activation, innate immune signaling and cell cycle regulation pathways.

NEK7 Antibody Jurkat Cell IP. Immunoprecipitation of NEK7 protein from 500 ug of human Jurkat whole cell lysate using 2 ug of NEK7 Antibody. Immunoprecipitated protein was analyzed by Western blot, with input lysate and rabbit IgG control samples included for comparison. NEK7 was detected at approximately 32 kDa, consistent with the predicted molecular weight of ~35 kDa. NEK7 Antibody / Inflammasome Activation Protein Antibody successfully enriches endogenous NIMA Related Kinase 7 protein and supports studies of NLRP3 inflammasome activation, protein interactions and innate immune signaling pathways.

NEK7 Antibody 293T Cell FACS. Flow cytometry testing of human 293T cells with NEK7 Antibody at 1 ug/million cells. Cells were fixed with 4% paraformaldehyde, permeabilized and blocked with 10% normal goat serum prior to intracellular staining. DyLight 488 conjugated goat anti-rabbit IgG was used as the secondary antibody. Red histogram represents blank control, green represents isotype control and blue represents NEK7 antibody staining. NEK7 Antibody / Inflammasome Activation Protein Antibody demonstrates detection of NIMA Related Kinase 7 expression and supports studies of NLRP3 inflammasome activation, innate immune signaling and cell cycle regulation pathways.

NEK7 Antibody Rat Bladder IHC. Immunohistochemistry staining of FFPE rat bladder tissue with NEK7 Antibody at 2 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-NEK7 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. NEK7 Antibody / Inflammasome Activation Protein Antibody demonstrates staining consistent with NIMA Related Kinase 7 expression and supports studies of NLRP3 inflammasome activation, innate immune signaling and inflammatory pathway regulation.

NEK7 Antibody Mouse Bladder IHC. Immunohistochemistry staining of FFPE mouse bladder tissue with NEK7 Antibody at 2 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-NEK7 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. NEK7 Antibody / Inflammasome Activation Protein Antibody demonstrates staining consistent with NIMA Related Kinase 7 expression and supports studies of NLRP3 inflammasome activation, innate immune signaling and inflammatory pathway regulation.