NEEP21 Antibody / Endosomal Recycling Protein Antibody (RQ8428)

NEEP21 Antibody Human Large B-Cell Lymphoma IHC. Immunohistochemistry was performed on FFPE human large B-cell lymphoma tissue using NEEP21 Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to antibody incubation. Bound antibody was detected using an HRP-conjugated secondary antibody with DAB chromogen. Diffuse cytoplasmic staining is observed throughout the neoplastic lymphoid cells, demonstrating endogenous NEEP21 expression within the tumor tissue. These results demonstrate that this Endosomal Recycling Protein Antibody is suitable for immunohistochemical detection of NEEP21 in formalin-fixed, paraffin-embedded human large B-cell lymphoma tissue.

NEEP21 Antibody Human Bladder Urothelial Carcinoma IHC. Immunohistochemistry was performed on FFPE human bladder urothelial carcinoma tissue using NEEP21 Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to antibody incubation. Bound antibody was detected using an HRP-conjugated secondary antibody with DAB chromogen. Moderate to strong cytoplasmic staining is observed throughout the neoplastic urothelial cells, consistent with endogenous expression of NEEP21, a protein involved in endosomal recycling and intracellular membrane trafficking. These results demonstrate that this Endosomal Recycling Protein Antibody is suitable for immunohistochemical detection of NEEP21 in formalin-fixed, paraffin-embedded human bladder urothelial carcinoma tissue.

NEEP21 Antibody Human Lung Cancer IHC. Immunohistochemistry was performed on FFPE human lung cancer tissue using NEEP21 Antibody. Heat-induced epitope retrieval was carried out in pH 8 EDTA buffer prior to antibody incubation. Bound antibody was detected using an HRP-conjugated secondary antibody with DAB chromogen. Moderate to strong cytoplasmic staining is observed throughout the neoplastic epithelial cells, consistent with endogenous expression of NEEP21, a protein that regulates endosomal recycling and intracellular membrane trafficking. These results demonstrate that this Endosomal Recycling Protein Antibody is suitable for immunohistochemical detection of NEEP21 in formalin-fixed, paraffin-embedded human lung cancer tissue.

NEEP21 Antibody Human, Rat and Mouse Brain WB. Western blot analysis was performed using NEEP21 Antibody. Lane 1: human SH-SY5Y cell lysate; lane 2: rat brain tissue lysate; lane 3: mouse brain tissue lysate. A specific immunoreactive band is detected at approximately 21 kDa in all three samples, consistent with the expected molecular weight of NEEP21. The blot demonstrates robust cross-species detection with minimal background, supporting reliable recognition of endogenous NEEP21 in neuronal cell and brain tissue lysates. These results demonstrate that this Endosomal Recycling Protein Antibody is suitable for Western blot detection of endogenous NEEP21 in human, rat, and mouse neural samples.

NEEP21 Antibody Human JK Cell FACS. Flow cytometry was performed on fixed and permeabilized human JK cells using NEEP21 Antibody at 1 ug/1×10^6 cells. Cells were blocked with normal goat serum prior to antibody incubation. Red: unstained cells; green: isotype control; blue: NEEP21 Antibody. A distinct rightward shift of the NEEP21-stained population relative to the isotype control demonstrates specific intracellular detection of endogenous NEEP21 expression in human JK cells. These results demonstrate that this Endosomal Recycling Protein Antibody is suitable for flow cytometric analysis of endogenous NEEP21 following fixation and permeabilization.