NEDD4 Antibody for IF / NEDD4 Immunofluorescence Antibody (RQ7237)

NEDD4 Antibody for IF Skeletal Muscle Tissue Immunofluorescence. Immunofluorescence analysis of Neural precursor cell expressed developmentally down-regulated protein 4 (NEDD4) in FFPE mouse skeletal muscle tissue using NEDD4 Antibody for IF. The antibody produces red cytoplasmic and membrane-associated staining (red) outlining muscle fiber architecture, consistent with NEDD4 localization in muscle cell compartments involved in protein turnover and membrane dynamics, while nuclei are counterstained with DAPI (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

NEDD4 Antibody Mouse Muscle Tissue IHC. Immunohistochemistry staining of FFPE mouse skeletal muscle tissue with NEDD4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

NEDD4 Antibody Brain IHC Rat Neuronal Cytoplasmic Staining. Immunohistochemistry analysis of Neural precursor cell expressed developmentally down-regulated protein 4 / NEDD4 in FFPE rat brain tissue using NEDD4 Antibody for IHC. HRP-DAB brown chromogenic staining demonstrates cytoplasmic localization within neuronal cell bodies distributed throughout the tissue, with surrounding neuropil showing lighter background signal. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

NEDD4 Antibody Rat Brain Tissue IHC Neuronal Distribution Pattern. Immunohistochemistry analysis of Neural precursor cell expressed developmentally down-regulated protein 4 / NEDD4 in FFPE rat brain tissue using NEDD4 Antibody for IHC. Brown HRP-DAB staining highlights scattered neuronal populations with distinct cell body labeling and minimal staining in non-neuronal regions, consistent with NEDD4 expression in neural tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

NEDD4 Antibody WB. Western blot testing of 1) rat L6, 2) mouse skeletal muscle, 3) mouse lung and 4) mouse B16-F10 cell lysate with NEDD4 antibody. Predicted molecular weight ~149 kDa; commonly observed at 110-149 kDa with a possible ~95 kDa cleavage band.

NEDD4 Antibody FACS. Flow cytometry testing of mouse HEPA1-6 cells with NEDD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NEDD4 antibody.