NEDD4 Antibody for IHC / NEDD4 Immunohistochemistry Antibody (RQ6891)

NEDD4 Antibody Renal Cell Carcinoma IHC Tumor Tissue. Immunohistochemistry analysis of Neural precursor cell expressed developmentally down-regulated protein 4 / NEDD4 in FFPE human renal cell carcinoma tissue using NEDD4 Antibody for IHC. HRP-DAB brown chromogenic staining demonstrates cytoplasmic and membrane-associated localization in tumor epithelial cells, with variable intensity across the tumor field, while surrounding stromal regions show comparatively reduced signal. The staining pattern highlights tumor cell architecture and supports evaluation of NEDD4 expression within the tumor microenvironment. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

NEDD4 Antibody Liver Cancer IHC Tumor Tissue. Immunohistochemistry analysis of Neural precursor cell expressed developmentally down-regulated protein 4 / NEDD4 in FFPE human liver cancer tissue using NEDD4 Antibody for IHC. HRP-DAB brown chromogenic staining demonstrates cytoplasmic and membrane-associated localization in tumor epithelial cells, with heterogeneous staining intensity across tumor regions and reduced signal in surrounding stromal compartments. The staining pattern highlights tumor cell morphology and supports evaluation of NEDD4 expression within hepatic tumor tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

NEDD4 Antibody Colon Adenocarcinoma IHC Tumor Architecture. Immunohistochemistry analysis of Neural precursor cell expressed developmentally down-regulated protein 4 / NEDD4 in FFPE human colon adenocarcinoma tissue using NEDD4 Antibody for IHC. HRP-DAB brown chromogenic staining demonstrates cytoplasmic and membrane-associated localization in gland-forming tumor epithelial cells, with variable staining intensity across malignant glands and reduced signal in stromal regions. The staining pattern highlights glandular tumor architecture and supports evaluation of NEDD4 expression in colorectal carcinoma tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

NEDD4 Antibody Lung Cancer IHC Tumor Tissue Analysis. Immunohistochemistry analysis of Neural precursor cell expressed developmentally down-regulated protein 4 / NEDD4 in FFPE human lung cancer tissue using NEDD4 Antibody for IHC. HRP-DAB brown chromogenic staining demonstrates cytoplasmic and membrane-associated localization in tumor epithelial cells, with heterogeneous staining across tumor regions and reduced signal in surrounding stromal areas. The staining pattern highlights tumor architecture and supports evaluation of NEDD4 expression within lung carcinoma tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

NEDD4 Antibody Rat Brain IHC Neuronal Cytoplasmic Staining. Immunohistochemistry analysis of Neural precursor cell expressed developmentally down-regulated protein 4 / NEDD4 in FFPE rat brain tissue using NEDD4 Antibody for IHC. HRP-DAB brown chromogenic staining demonstrates cytoplasmic localization in neuronal cell bodies distributed throughout the tissue, with lighter background signal in surrounding neuropil and minimal staining in non-neuronal regions. The staining pattern supports neuronal expression of NEDD4 within brain tissue architecture. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

NEDD4 Antibody A549 Cells IF. Immunofluorescent staining of FFPE human A549 cells with NEDD4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

NEDD4 Antibody WB. Western blot testing of human 1) U-251, 2) RT-4 and 3) HaCat cell lysate with NEDD4 antibody. Predicted molecular weight ~149 kDa; commonly observed at 110-149 kDa with a possible ~95 kDa cleavage band.

NEDD4 Antibody U-87 MG Cells FACS. Flow cytometry testing of human U-87 MG cells with NEDD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NEDD4 antibody.