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Home >> Antibodies >> NEDD4-2 Antibody / NEDD4L

NEDD4-2 Antibody / NEDD4L (RQ4197)

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  Catalog No Formulation Size Price (USD)  
Image RQ4197 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human 22RV1, 2) human Jurkat, 3) rat pancreas, 4) mouse pancreas and 5) mouse NIH3T3 lysate with NEDD4-2 antibody at 0.5ug/ml. Predicted molecular weight: 112-125 kDa.
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Western blot testing of 1) human 22RV1, 2) human Jurkat, 3) rat pancreas, 4) mouse pancreas and 5) mouse NIH3T3 lysate with NEDD4-2 antibody at 0.5ug/ml. Predicted molecular weight: 112-125 kDa.
Flow cytometry testing of human U-2 OS cells with NEDD4-2 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NEDD4-2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q96PU5
Applications Western Blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NEDD4-2 antibody is available for research use only.
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Description

Neural precursor cell expressed developmentally downregulated gene 4-like (NEDD4L) or NEDD4-2 (NEDD4-2) is an enzyme (ubiquitin ligase) of the NEDD4 family. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Application Notes

Optimal dilution of the NEDD4-2 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids N703-D975 was used as the immunogen for the NEDD4-2 antibody.

Storage

After reconstitution, the NEDD4-2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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