Nectin cell adhesion molecule 4 Antibody / NECTIN4 [clone NECTIN4/9906] (V6025)

Immunohistochemistry analysis of Nectin cell adhesion molecule 4 / NECTIN4 antibody (clone NECTIN4/9906) in human skin tissue. Formalin-fixed, paraffin-embedded skin demonstrates strong membranous HRP-DAB brown staining within the epidermal keratinocyte layer, outlining cell-cell borders consistent with surface localization of Nectin-4. The underlying dermis shows minimal staining, supporting epithelial-restricted expression. Hematoxylin counterstain highlights tissue morphology and nuclei. The inset shows PBS used in place of primary antibody as a negative control, confirming absence of non-specific secondary antibody binding. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.

Immunohistochemistry analysis of Nectin cell adhesion molecule 4 / NECTIN4 antibody (clone NECTIN4/9906) in human placental tissue. Formalin-fixed, paraffin-embedded placenta demonstrates membranous HRP-DAB brown staining outlining trophoblastic cells within chorionic villi, consistent with surface localization of Nectin-4. The staining pattern highlights cell-cell borders with minimal background in stromal regions. Hematoxylin counterstain delineates nuclear morphology and villous architecture. The inset shows PBS used in place of primary antibody as a negative control, confirming absence of non-specific secondary antibody binding. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.

Western blot analysis of Nectin cell adhesion molecule 4 / NECTIN4 antibody (clone NECTIN4/9906) in human A-431 and MCF7 cell lysates. A distinct band is detected at approximately 60-70 kDa in both lanes, consistent with the predicted molecular weight of Nectin-4, a glycosylated type I transmembrane protein. The observed migration slightly above the core predicted size is consistent with known N-linked glycosylation of NECTIN4. Band intensity is comparable between A-431 and MCF7 cells, supporting expression in epithelial-derived tumor cell lines.

Protein microarray specificity analysis of Nectin cell adhesion molecule 4 / NECTIN4 antibody (clone NECTIN4/9906). The antibody was screened against a protein array containing more than 19,000 full-length human proteins. PVRL4 ranked as the top hit with a Z-score of 105.73 and an S-score of 79.68, indicating highly specific recognition of the intended target. The next highest signals, including DNAI2_frag and SHFM1, showed substantially lower Z-scores and minimal S-scores, supporting target selectivity. The Z-score reflects the signal strength generated when the monoclonal antibody, in combination with a fluorescently labeled anti-IgG secondary antibody, binds to a specific protein on the array. Z-scores are expressed as standard deviations above the mean signal intensity across the array. The S-score represents the difference between the Z-score of the top-ranked protein and the next highest ranked protein, providing a measure of relative specificity. An S-score of 2.5 or greater is generally considered indicative of target-specific binding. The high S-score observed for PVRL4 demonstrates strong specificity of clone NECTIN4/9906 for Nectin-4.

SDS-PAGE Analysis of Purified Nectin cell adhesion molecule 4/NECTIN4 antibody (NECTIN4/9906). Confirmation of Purity and Integrity of Antibody.