NEAS Antibody / Neural Cytoskeleton Marker (RQ6726)

NEAS Antibody Human HeLa Cervical Cancer Cell IF. Immunofluorescence of FFPE human HeLa cervical cancer cells using NEAS Antibody shows cytoplasmic green fluorescence consistent with SPTAN1 localization along the submembranous cytoskeleton and cellular extensions. Nuclei were counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 min prior to staining. This NEAS Antibody demonstrates detection of the spectrin-based cytoskeleton in human cells.

NEAS Antibody Human Hashimoto's Thyroiditis IHC. Immunohistochemistry of FFPE human Hashimoto's thyroiditis tissue using NEAS Antibody shows prominent HRP-DAB brown staining outlining thyroid follicular epithelial cells, consistent with the submembranous localization of SPTAN1/NEAS. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining. This NEAS Antibody demonstrates detection of the spectrin-based cytoskeleton in human thyroid tissue.

NEAS Antibody Human Testis Large B Cell Tumor IHC. Immunohistochemistry of FFPE human testis large B cell tumor tissue using NEAS Antibody shows HRP-DAB brown staining within tumor cells, consistent with cytoplasmic and submembranous localization of SPTAN1/NEAS. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining. This NEAS Antibody demonstrates detection of the spectrin-based cytoskeleton in human tumor tissue.

NEAS Antibody Human Thyroid Papillary Carcinoma IHC. Immunohistochemistry of FFPE human thyroid papillary carcinoma tissue using NEAS Antibody shows HRP-DAB brown staining outlining tumor epithelial cells, consistent with the submembranous localization of SPTAN1/NEAS. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining. This NEAS Antibody demonstrates detection of the spectrin-based cytoskeleton in human thyroid papillary carcinoma tissue.

NEAS Antibody Mouse Brain IHC. Immunohistochemistry of FFPE mouse brain tissue using NEAS Antibody / Neural Cytoskeleton Marker shows diffuse HRP-DAB brown staining throughout the neuropil with staining of neuronal cell bodies, consistent with the broad distribution of SPTAN1/NEAS in the neural cytoskeleton. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining. This NEAS Antibody demonstrates detection of the neural cytoskeleton marker in mouse brain tissue.

NEAS Antibody Rat Brain IHC. Immunohistochemistry of FFPE rat brain tissue using NEAS Antibody / Neural Cytoskeleton Marker shows diffuse HRP-DAB brown staining throughout the neuropil with staining of neuronal cell bodies, consistent with the broad distribution of SPTAN1/NEAS in the neural cytoskeleton. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining. This NEAS Antibody demonstrates detection of the neural cytoskeleton marker in rat brain tissue.

NEAS Antibody Mouse and Rat Lung and Brain WB. Western blot analysis using NEAS Antibody / Neural Cytoskeleton Marker was performed on 1) rat lung, 2) rat brain, 3) mouse lung, 4) mouse brain and 5) mouse NIH 3T3 cell lysates. The antibody detects a prominent band near the predicted molecular weight of approximately 284 kDa. Detection across brain and non-neural samples is consistent with the broad tissue distribution of SPTAN1/NEAS. This NEAS Antibody supports detection of the neural cytoskeleton marker in mouse and rat samples.

NEAS Antibody Human Caco-2 Colorectal Cancer Cell FACS. Flow cytometry of human Caco-2 colorectal cancer cells using NEAS Antibody at 1 ug/million cells following blocking with goat sera. The histogram shows cells alone (red), isotype control (green) and NEAS Antibody staining (blue). The rightward shift of the antibody-stained population relative to the isotype control demonstrates detection of SPTAN1/NEAS in Caco-2 cells. This NEAS Antibody supports detection of the spectrin-based cytoskeleton in human cells.