NDST1 Antibody / N-deacetylase/N-sulfotransferase 1 (FY12617)

Western blot analysis of NDST1 using anti-NDST1 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human HUVEC whole cell lysates. Lane 2: human whole cell lysates. Lane 3: human CACO-2 whole cell lysates. Lane 4: rat heart tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDST1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substratewith Tanon 5200 system. Western blot probed with anti-NDST1 shows a predominant band at ~70 kDa, lower than the predicted ~101 kDa, consistent with truncated or proteolytically processed forms of the Golgi-localized N-deacetylase/N-sulfotransferase 1.