NDPK-B Antibody / Nucleoside diphosphate kinase B / NME2 [clone NME2/6436] (V5771)

IHC analysis of NDPK-B / NME2 antibody in human kidney tissue. Formalin-fixed, paraffin-embedded human kidney shows cytoplasmic HRP-DAB brown chromogenic staining predominantly in renal tubular epithelial cells, while glomerular structures exhibit minimal staining. Clone NME2/6436 demonstrates specific signal following heat-induced epitope retrieval in pH 9 10mM Tris with 1mM EDTA for 20 min. The inset negative control using PBS instead of primary antibody shows no specific staining.

Western blot testing of human 293T and MCF7 cell lysate with NDPK-B antibody (clone NME2/6436). Predicted molecular weight ~17 kDa.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using NDPK-B antibody (clone NME2/6436). These results demonstrate the foremost specificity of the NME2/6436 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free NDPK-B antibody (clone NME2/6436) as confirmation of integrity and purity.