- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
NDC80 Antibody / HEC1 (RQ5648)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ5648 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Purity | Affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide |
| UniProt | O14777 |
| Localization | Nuclear |
| Applications | Western Blot : 0.5-1ug/ml Immunofluorescence : 2-4ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml |
| Limitations | This NDC80 antibody is available for research use only. |
|
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NDC80 antibody detects NDC80 (also known as HEC1), a core kinetochore protein essential for chromosome segregation during mitosis and meiosis. The UniProt recommended name is Kinetochore protein NDC80 homolog (NDC80). This conserved coiled-coil protein forms part of the NDC80 complex, which anchors spindle microtubules to kinetochores and ensures accurate chromosome alignment and segregation. NDC80 is crucial for maintaining genomic stability during cell division and preventing aneuploidy.
NDC80 functions as one of four core components of the outer kinetochore complex, along with NUF2, SPC24, and SPC25. These proteins assemble into a rod-shaped structure that extends outward from the centromere, forming the primary attachment site for microtubule plus-ends. The N-terminal domain of NDC80 binds directly to microtubules through calponin homology motifs, while the C-terminal region interacts with SPC25 and NUF2 to stabilize the entire complex. This microtubule-binding activity is regulated by phosphorylation of key serine and threonine residues by Aurora B kinase, which adjusts kinetochore-microtubule attachment strength throughout mitosis.
The NDC80 gene is located on chromosome 1q42.13 and encodes a protein of approximately 74 kDa. NDC80 expression is tightly regulated through the cell cycle, peaking in mitotic cells. It localizes predominantly to kinetochores during metaphase and early anaphase and disperses as cells exit mitosis. Its expression level and localization serve as markers for proliferating cells and accurate spindle checkpoint control. Misexpression or mutation of NDC80 disrupts kinetochore structure and microtubule attachment, leading to chromosome missegregation and genomic instability, phenomena frequently observed in tumor cells.
Beyond its role in chromosome segregation, NDC80 participates in mitotic checkpoint signaling by recruiting regulatory proteins such as MAD1, MAD2, and BUB1 to the kinetochore. It acts as a scaffold for spindle checkpoint assembly, ensuring that anaphase onset is delayed until all kinetochores achieve proper microtubule attachment. Dysregulation of NDC80 has been reported in several cancers, including breast, lung, and hepatocellular carcinomas, where overexpression correlates with high proliferative indices and poor clinical prognosis.
NDC80 antibody is useful for detecting NDC80 expression in dividing cells and for studying kinetochore architecture, mitotic progression, and checkpoint signaling. It serves as a reliable marker of proliferative activity in cultured cells and tissue sections. NSJ Bioreagents provides NDC80 antibody validated for use in relevant research applications supporting studies in mitosis, chromosomal dynamics, and cell cycle regulation.
Optimal dilution of the NDC80 antibody should be determined by the researcher.
Recombinant human protein (amino acids N87-E602) was used as the immunogen for the NDC80 antibody.
After reconstitution, the NDC80 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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