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Home >> Antibodies >> NCOA5 Antibody / Nuclear receptor coactivator 5

NCOA5 Antibody / Nuclear receptor coactivator 5 (RQ8361)

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  Catalog No Formulation Size Price (USD)  
Image RQ8361 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human HeLa cells with NCOA5 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with NCOA5 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human HeLa, 3) rat brain, 4) mouse brain and 5) mouse thymus tissue lysate with NCOA5 antibody. Predicted molecular weight ~66 kDa.
Flow cytometry testing of fixed and permeabilized human 293T cells with NCOA5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCOA5 antibody.
IHC staining of FFPE mouse brain tissue with NCOA5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with NCOA5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with NCOA5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal cancer tissue with NCOA5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with NCOA5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9HCD5
Localization Nuclear
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This NCOA5 antibody is available for research use only.
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Description

Nuclear receptor coactivator 5 (NCOA5), also known as coactivator independent of AF-2 function (CIA), is a protein that in humans is encoded by the NCOA5 gene. This gene encodes a coregulator for the alpha and beta estrogen receptors and the orphan nuclear receptor NR1D2. The protein localizes to the nucleus, and is thought to have both coactivator and corepressor functions. Its interaction with nuclear receptors is independent of the AF2 domain on the receptors, which is known to regulate interaction with other coreceptors. Several alternatively spliced transcript variants have been found for this gene.

Application Notes

Optimal dilution of the NCOA5 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (R191-Q560) was used as the immunogen for the NCOA5 antibody.

Storage

After reconstitution, the NCOA5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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