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Home >> Antibodies >> NCL Antibody / Nucleolin

NCL Antibody / Nucleolin [clone MSVA-623R] (V6101)

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  Catalog No Formulation Size Price (USD)  
Image V6101-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 654.50
Image
V6101-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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Nucleolin Rabbit Recombinant Monoclonal Antibody (MSVA-623R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas. <br><br>Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Nucleolin Rabbit Recombinant Monoclonal Antibody (MSVA-623R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-623R
UniProt P19338
Localization Cytoplasm, Nucleolus, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This NCL/Nucleolin antibody is available for research use only.
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Application Notes

1. Optimal dilution of the NCL/Nucleolin antibody should be determined by the researcher.

2. This NCL/Nucleolin antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

Recombinant full-length human NCL protein was used as the immuongen for the NCL/Nucleolin antibody.

Storage

NCL/Nucleolin antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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