NCL Antibody for IF / Nucleolin Immunofluorescence Antibody [clone 364-5] (V2757)

Description

Nucleolin (NCL) is a highly conserved nucleolar protein that functions as a central regulator of ribosomal RNA synthesis, ribosome assembly, and nucleolar organization. The protein is encoded by the NCL gene on chromosome 2q37 and is one of the most abundant phosphoproteins present in the nucleolus of actively proliferating cells. Nucleolin participates in the transcription and processing of ribosomal RNA, stabilization of specific messenger RNAs, and regulation of chromatin architecture. The NCL Antibody for IF clone 364-5 is a mouse monoclonal reagent developed for visualization of nucleolin localization by immunofluorescence microscopy, enabling investigation of nucleolar structure and RNA regulatory processes.

Nucleolin contains several structural domains that support its multifunctional roles in nucleic acid metabolism. These include an acidic N-terminal domain involved in chromatin interactions, four RNA recognition motifs responsible for binding ribosomal RNA and other transcripts, and a glycine- and arginine-rich C-terminal region that facilitates protein-protein interactions. Through these domains, nucleolin coordinates multiple steps of ribosome production and contributes to the structural organization of nucleoli. Because of its nucleolar enrichment and characteristic punctate nuclear pattern, a well-characterized Nucleolin Immunofluorescence Antibody can be used to monitor nucleolar morphology and changes in nucleolar integrity during cellular stress or cell cycle progression.

NCL antibody reagents are frequently described in the literature using several established aliases for the protein. NCL antibody, also known as nucleolin antibody, C23 antibody, and nucleolar protein NCL antibody, recognizes a protein historically described as nucleolin nucleolar phosphoprotein. The C23 name originates from early nucleolar fractionation studies in which nucleolin was identified as a prominent nucleolar phosphoprotein associated with ribosomal transcription complexes. These historical designations remain common in studies of nucleolar biology, RNA processing, and cancer cell proliferation.

Nucleolin exhibits dynamic intracellular trafficking and can shuttle between the nucleolus, nucleoplasm, cytoplasm, and in some conditions the cell surface. In proliferating and transformed cells, nucleolin levels are often elevated and correlate with increased ribosome production and enhanced protein synthesis. Surface-localized nucleolin has also been reported in tumor cells and activated endothelial cells, where it participates in ligand binding and signaling pathways linked to cell growth and angiogenesis. These functions make nucleolin an important subject of investigation in cancer biology and nucleolar stress responses.

The protein also contributes to regulation of DNA replication and chromatin remodeling through interactions with histones, transcription factors, and RNA-binding complexes. By coordinating ribosomal RNA transcription with ribosome assembly, nucleolin helps ensure efficient protein synthesis in rapidly dividing cells. Alterations in nucleolin localization or expression are therefore frequently associated with tumor progression and cellular stress responses.

Because nucleolin accumulates strongly within nucleoli and displays a distinctive nuclear distribution pattern, antibodies targeting NCL are widely used as markers of nucleolar compartments in cell imaging experiments. A mouse monoclonal NCL Antibody for IF such as clone 364-5 can support immunofluorescence analysis of nucleolar organization, assessment of nucleolar disruption following drug treatment, and visualization of nucleolin redistribution during cellular stress or apoptosis.

Application Notes

Optimal dilution of the NCL Antibody for IF should be determined by the researcher.

1.

Immunogen

SUDHL1 cell nuclear lysate was used as the immunogen for the NCL/Nucleolin antibody.

Storage

Store the NCL/Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

nucleolin antibody, C23 antibody, nucleolar protein NCL antibody, nucleolin nucleolar phosphoprotein antibody