- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Nucleolar Protein Nucleolin Antibody for IF / NCL Immunofluorescence Antibody (F44030)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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F44030-0.4ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.4 ml | 439 | |
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F44030-0.08ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.08 ml | 205 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Predicted Reactivity | Primate |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity |
| UniProt | P19338 |
| Applications | Western Blot : 1:1000 Immunofluorescence : 1:10-1:50 |
| Limitations | This NCL/Nucleolin antibody is available for research use only. |
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Nucleolin (NCL) is a highly abundant nucleolar protein that plays a central role in ribosome biogenesis, ribosomal RNA processing, and nucleolar organization. The protein is encoded by the NCL gene on chromosome 2q37 and is widely expressed in proliferating cells where high levels of ribosome production are required. Nucleolin participates in transcription of ribosomal RNA genes, processing of pre-rRNA transcripts, and assembly of ribonucleoprotein complexes within the nucleolus. The Nucleolar Protein Nucleolin Antibody for IF is a rabbit polyclonal reagent developed to visualize nucleolin localization using immunofluorescence microscopy, supporting investigation of nucleolar structure and RNA regulatory pathways.
Nucleolin contains several functional domains that enable its diverse biological activities. The N-terminal acidic domain interacts with chromatin and nucleolar components involved in ribosomal RNA transcription. The central portion of the protein contains multiple RNA recognition motifs that bind ribosomal RNA and other RNA transcripts. A glycine- and arginine-rich C-terminal region facilitates interactions with nucleic acids and nucleolar proteins. Through these structural features, nucleolin helps coordinate ribosomal RNA synthesis, ribosome assembly, and nucleolar organization. Because nucleolin accumulates prominently within nucleoli, a well-characterized NCL Immunofluorescence Antibody is commonly used to visualize nucleolar compartments and assess nucleolar integrity during cellular stress or cell cycle progression.
NCL antibody reagents are frequently described in the literature using several established synonyms for the protein. NCL antibody, also known as nucleolin antibody, C23 nucleolin antibody, and nucleolin nucleolar phosphoprotein antibody, recognizes a major nucleolar phosphoprotein originally identified in nucleolar protein fractionation studies. The C23 designation derives from early biochemical characterization in which nucleolin was classified as a prominent nucleolar phosphoprotein associated with ribosomal RNA transcription complexes. These alternative names remain widely used in research focused on nucleolar biology and ribosome synthesis.
Nucleolin is dynamically distributed within cells and can shuttle between the nucleolus, nucleoplasm, and cytoplasm depending on cellular conditions. In rapidly dividing cells, nucleolin levels are typically elevated to support increased ribosome production and protein synthesis. Elevated expression of nucleolin is frequently observed in tumor cells and other proliferative tissues, where it contributes to enhanced ribosomal RNA transcription and regulation of RNA stability. In certain contexts nucleolin has also been detected at the cell surface, where it can participate in ligand binding and signaling pathways linked to cell growth and angiogenesis.
In addition to its role in ribosome biogenesis, nucleolin participates in regulation of DNA replication, chromatin remodeling, and RNA transport. The protein interacts with histones, transcription factors, and RNA-binding proteins to coordinate nucleolar activity with broader nuclear regulatory processes. These interactions allow nucleolin to influence gene expression programs and cellular stress responses, particularly during conditions that disrupt nucleolar function.
Because nucleolin displays a characteristic nucleolar enrichment and punctate nuclear fluorescence pattern, antibodies targeting NCL are widely used as markers of nucleolar compartments in microscopy studies. A rabbit polyclonal Nucleolar Protein Nucleolin Antibody for IF can therefore support immunofluorescence analysis of nucleolar organization, investigation of nucleolar stress responses, and visualization of nucleolin redistribution during cell cycle progression or cellular stress.
Titration of the Nucleolar Protein Nucleolin Antibody for IF may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 276-304 from the human protein was used as the immunogen for this NCL antibody.
Aliquot the NCL/Nucleolin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
NCL antibody, nucleolin antibody, C23 nucleolin antibody, nucleolin nucleolar phosphoprotein antibody
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