NCAM1 Antibody / Neural Cell Adhesion Molecule 1 Antibody (RQ5592)

NCAM1 Antibody U2OS Cell IF. Immunofluorescent staining of human U2OS cells demonstrates NCAM1 expression following enzyme antigen retrieval and blocking with 10% goat serum. Cells were incubated with NCAM1 Antibody at 5 ug/ml overnight at 4°C, followed by Cy3-conjugated secondary antibody. NCAM1 staining is shown in red with DAPI nuclear counterstaining in blue. This Neural Cell Adhesion Molecule 1 Antibody supports studies of neural cell adhesion, cell-cell interactions, and NCAM1 expression and localization.

NCAM1 Antibody Human, Rat and Mouse WB. Western blot analysis demonstrates NCAM1 expression in human SH-SY5Y, U2OS, U251 and HEL cells, rat brain and C6 cells, and mouse brain. NCAM1 Antibody was used at 0.25 ug/ml. Multiple immunoreactive bands are detected at approximately 120–250 kDa, consistent with the known molecular heterogeneity of NCAM1 resulting from alternative splicing and extensive post-translational modification. This Neural Cell Adhesion Molecule 1 Antibody supports studies of neural cell adhesion, neuronal development, cell-cell interactions, and nervous system biology.

NCAM1 Antibody Human Cerebellum IHC. Immunohistochemical staining of FFPE human cerebellum tissue demonstrates NCAM1 expression following heat-induced epitope retrieval in pH8 EDTA buffer. Sections were blocked with 10% goat serum and incubated with NCAM1 Antibody at 2 ug/ml overnight at 4°C, followed by HRP/DAB detection. Brown staining is observed across the cerebellar tissue, with hematoxylin counterstaining in blue. This Neural Cell Adhesion Molecule 1 Antibody supports studies of neural cell adhesion, neuronal development, and cerebellar biology.

NCAM1 Antibody Human Cerebral Cortex IHC. Immunohistochemical staining of FFPE human cerebral cortex tissue demonstrates NCAM1 expression following heat-induced epitope retrieval in pH8 EDTA buffer. Sections were blocked with 10% goat serum and incubated with NCAM1 Antibody at 2 ug/ml overnight at 4°C, followed by HRP/DAB detection. Brown staining is observed throughout the neural tissue, with hematoxylin counterstaining in blue. This Neural Cell Adhesion Molecule 1 Antibody supports studies of neural cell adhesion, neuronal development, and nervous system biology.