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Home >> Antibodies >> NCAM1 Antibody / Neural cell adhesion molecule 1

NCAM1 Antibody / Neural cell adhesion molecule 1 [clone MSVA-056R] (V6100)

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  Catalog No Formulation Size Price (USD)  
Image V6100-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 654.50
Image
V6100-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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Neural cell adhesion molecule 1 Rabbit Recombinant Monoclonal Antibody (MSVA-056R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-056R
UniProt P13591 , P13592
Localization Cell membrane, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This NCAM1/Neural cell adhesion molecule 1 antibody is available for research use only.
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Application Notes

1. Optimal dilution of the NCAM1/Neural cell adhesion molecule 1 antibody should be determined by the researcher.

2. This NCAM1/Neural cell adhesion molecule 1 antibody is recombinantly produced by expression in CHO cells.

Immunogen

A recombinant fragment (around amino acids 200-400) of human NCAM1 (CD56) protein (exact sequence is proprietary) was used as the immuongen for the NCAM1/Neural cell adhesion molecule 1 antibody.

Storage

NCAM1/Neural cell adhesion molecule 1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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