- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
NARC1 Antibody / PCSK9 Polyclonal Antibody (RQ6387)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ6387 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
|
| Availability | 1-3 business days |
| Species Reactivity | Mouse, Rat |
| Format | Purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q80W65 |
| Applications | Western blot: 0.5-1ug/ml Direct ELISA: 0.1-0.5ug/ml |
| Limitations | This NARC1 Antibody / PCSK9 Polyclonal Antibody is available for research use only. |
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NARC1 Antibody / PCSK9 Polyclonal Antibody targets neural apoptosis-regulated convertase 1 (NARC1), now commonly known as proprotein convertase subtilisin/kexin type 9 (PCSK9). This protein belongs to the subtilisin-like serine protease family and plays an important role in regulating cholesterol metabolism through its effects on low-density lipoprotein receptor (LDLR) abundance. Although initially identified in studies of neuronal differentiation and apoptosis, PCSK9 is now best characterized as a regulator of circulating low-density lipoprotein cholesterol. Its expression is particularly prominent in the liver, where it influences the efficiency of LDL particle clearance from the bloodstream.
PCSK9 is synthesized as an inactive precursor containing an N-terminal signal peptide, a prodomain, a catalytic domain, and a C-terminal cysteine- and histidine-rich domain. During maturation in the endoplasmic reticulum, PCSK9 undergoes autocatalytic cleavage between its prodomain and catalytic domain. The cleaved prodomain remains tightly associated with the mature protein, preventing conventional proteolytic activity while supporting proper folding and secretion. This unusual maturation mechanism distinguishes PCSK9 from many other members of the proprotein convertase family.
Following secretion, PCSK9 binds the epidermal growth factor-like repeat A domain of LDLR. This interaction promotes the delivery of internalized receptors to lysosomes, reducing their recycling to the cell surface. Consequently, elevated PCSK9 activity decreases hepatic LDLR availability and limits the removal of circulating LDL particles. PCSK9 expression is regulated in part by sterol regulatory element-binding protein 2 (SREBP2), which coordinates the transcription of genes involved in cholesterol synthesis and uptake. This creates a feedback relationship between cellular sterol availability, LDLR production, and receptor degradation.
Genetic variation in PCSK9 has provided important evidence of its physiological significance. Gain-of-function mutations can cause autosomal dominant hypercholesterolemia, whereas loss-of-function variants are associated with reduced LDL cholesterol and lower cardiovascular disease risk. These findings established PCSK9 as a therapeutic target and contributed to the development of monoclonal antibodies and RNA-based treatments that reduce its activity or production. PCSK9 has also been investigated in relation to hepatic metabolism, inflammatory signaling, and neuronal biology, although its contributions outside cholesterol regulation remain less fully understood. This NARC1 Antibody provides an additional reagent for investigating PCSK9 expression and its role in lipoprotein metabolism and cholesterol homeostasis.
Explore our PCSK9 Antibody for additional research into PCSK9 expression, LDL receptor regulation, and cholesterol metabolism, with Western blot validation using HeLa wild-type and PCSK9 knockout cell lysates.
Optimal dilution of the NARC1 Antibody / PCSK9 Polyclonal Antibody should be determined by the researcher.
Recombinant mouse protein (amino acids Q182-H560) was used as the immunogen for the NARC1 Antibody.
After reconstitution, the NARC1 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Neural apoptosis-regulated convertase 1 antibody, Neural apoptosis-regulated convertase-1 antibody, NARC-1 antibody, Proprotein convertase subtilisin/kexin type 9 antibody, Proprotein convertase 9 antibody, PC9 antibody, PCSK9 protein antibody, Subtilisin-like proprotein convertase 9 antibody
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![Western blot of HEK293 lysate overexpressing human PCSK9 with MYC tag probed with PCSK9 antibody [1ug/ml] in Lane A and probed with anti-MYC tag [1/1000] in lane C. Mock-transfected HEK293 probed with PCSK9 antibody [1ug/ml] in Lane B.](https://www.nsjbio.com/plp/thumb_image/img-R36265-100UG-1.jpg?v=1794750603 "Western blot of HEK293 lysate overexpressing human PCSK9 with MYC tag probed with PCSK9 antibody [1ug/ml] in Lane A and probed with anti-MYC tag [1/1000] in lane C. Mock-transfected HEK293 probed with PCSK9 antibody [1ug/ml] in Lane B.")
