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Home >> Antibodies >> Napsin A Antibody

Napsin A Antibody [clone NPSNA-1] (V7177)

  Catalog No Formulation Size Price (USD)  
Image V7177-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V7177-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V7177SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
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V7177IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 499
Western blot of 1) K562, 2) 293 and 3) A549 cell lysates using Napsin A antibody (clone NPSNA-1). Predicted molecular weight ~45 kDa.
IHC testing of FFPE human lung adenocarcinoma with Napsin A antibody (clone NPSNA-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name NPSNA-1
Purity Protein G affinity chromatography
UniProt O96009
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This Napsin A antibody is available for research use only.
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Description

Napsin-A is a protein that in humans is encoded by the NAPSA gene. The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pro-Napsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [Wiki]

Application Notes

Titering of the Napsin A antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids 189-299 from the human protein were used as the immunogen for the Napsin A antibody.

Storage

Store the Napsin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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