Napsin A Antibody / Protein Microarray Validated Antibody [clone NAPSA/3309] (V8243)

Napsin A Antibody Lung Adenocarcinoma IHC. Immunohistochemistry analysis of Napsin A (NAPSA) expression in FFPE human lung adenocarcinoma demonstrates granular cytoplasmic HRP-DAB brown staining in tumor epithelial cells with minimal background in surrounding stromal regions. Clone NAPSA/3309, a protein microarray validated mouse monoclonal antibody, supports selective detection of Napsin A consistent with its role as a pulmonary epithelial differentiation marker. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes followed by cooling at RT for 20 minutes prior to staining.

Napsin A Antibody Protein Microarray Validation. Analysis of Napsin A (NAPSA) specificity using a human protein microarray containing more than 19,000 full-length human proteins demonstrates strong and selective binding of clone NAPSA/3309 to its intended target, with NAPSA ranked as the top hit by signal intensity. The Z-score reflects the strength of binding relative to the array-wide mean signal, expressed as standard deviations above background, while the S-score represents the difference in Z-scores between the top-ranked target and the next highest protein, indicating relative specificity. The high Z- and S-scores observed for NAPSA support selective target recognition with minimal cross-reactivity across the proteome-scale panel.

SDS-PAGE analysis of purified, BSA-free Napsin A antibody (clone NAPSA/3309) as confirmation of integrity and purity.