Napsin A Antibody / Pulmonary Epithelial Marker Antibody [clone NAPSA/3308] (V7604)

Napsin A Antibody for IHC. Immunohistochemistry analysis of Napsin A (NAPSA) expression in FFPE human lung adenocarcinoma demonstrates granular cytoplasmic HRP-DAB brown staining in tumor epithelial cells with minimal background in surrounding stromal regions. Clone NAPSA/3308 highlights pulmonary epithelial cell populations consistent with the role of Napsin A as a lung epithelial marker, supporting identification of epithelial lineage within tumor tissue. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes followed by cooling at RT for 20 minutes prior to staining.

Napsin A Antibody Protein Microarray Validation. Analysis of Napsin A (NAPSA) specificity using a human protein microarray containing more than 19,000 full-length human proteins demonstrates strong and selective binding of clone NAPSA/3308 to its intended target, with NAPSA ranked as the top hit by signal intensity. The Z-score reflects binding strength relative to the array-wide mean signal, expressed as standard deviations above background, while the S-score represents the difference between the top-ranked target and the next highest signal, indicating relative specificity. The high Z- and S-scores observed for NAPSA support selective target recognition with minimal cross-reactivity across a proteome-scale panel.

SDS-PAGE analysis of purified, BSA-free Napsin A antibody (clone NAPSA/3308) as confirmation of integrity and purity.