Napsin A Antibody / Alveolar Differentiation Marker Antibody [clone NAPSA/3307] (V8481)

Napsin A Antibody for IHC. Immunohistochemistry analysis of Napsin A (NAPSA) expression in FFPE human lung adenocarcinoma demonstrates strong granular cytoplasmic HRP-DAB brown staining in tumor epithelial cells with minimal background in surrounding stromal regions. Clone NAPSA/3307 highlights alveolar epithelial differentiation consistent with expression in type II pneumocyte-derived cells, supporting identification of alveolar lineage within tumor tissue. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining.

IHC staining of FFPE human lung adenocarcinoma with Napsin A antibody (clone NAPSA/3307). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Napsin A Antibody Protein Microarray Validation. Analysis of Napsin A (NAPSA) specificity using a human protein microarray containing more than 19,000 full-length human proteins demonstrates selective binding of clone NAPSA/3307 to its intended target, with NAPSA ranked as the top signal by Z-score. The Z-score reflects binding strength relative to the array-wide mean signal, expressed as standard deviations above background, while the S-score represents the difference between the top-ranked target and the next highest signal, indicating relative specificity. The observed Z- and S-scores support strong target recognition of Napsin A with limited cross-reactivity across a proteome-scale panel.

SDS-PAGE analysis of purified, BSA-free Napsin A antibody (clone NAPSA/3307) as confirmation of integrity and purity.