Napsin A Antibody / HuProt Microarray Validated Antibody [clone NAPSA/3306] (V8244)

Napsin A Antibody for IHC. Immunohistochemistry analysis of Napsin A (NAPSA) expression in FFPE human lung adenocarcinoma demonstrates distinct granular cytoplasmic HRP-DAB brown staining in tumor epithelial cells, with minimal background in surrounding stromal tissue. Clone NAPSA/3306, a HuProt microarray validated mouse monoclonal antibody, supports highly specific detection of Napsin A consistent with its role as a pulmonary epithelial differentiation marker. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes followed by cooling at RT for 20 minutes prior to staining.

Napsin A Antibody HuProt Microarray Validation. Analysis of Napsin A (NAPSA) specificity using a HuProt microarray containing more than 19,000 full-length human proteins demonstrates highly selective binding of clone NAPSA/3306 to its intended target, with NAPSA ranked as the top signal by Z-score. The Z-score reflects binding strength relative to the array-wide mean signal, expressed as standard deviations above background, while the S-score represents the difference between the top-ranked target and the next highest signal, indicating relative specificity. The high Z- and S-scores observed for NAPSA support proteome-wide specificity with minimal cross-reactivity across a large panel of human proteins.