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Home >> Antibodies >> Napsin A Antibody

Napsin A Antibody [clone NAPSA/3306] (V8244)

  Catalog No Formulation Size Price (USD)  
Image V8244-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8244-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8244SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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IHC staining of FFPE human lung adenocarcinoma with Napsin A antibody (clone NAPSA/3306). HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Napsin A antibody (clone NAPSA/3306). These results demonstrate the foremost specificity of the NAPSA/3306 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name NAPSA/3306
Purity Protein G affinity chromatography
UniProt O96009
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Napsin A antibody is available for research use only.
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Description

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Application Notes

Optimal dilution of the Napsin A antibody should be determined by the researcher.

Immunogen

Amino acids 189-299 from the human protein were used as the immunogen for the Napsin A antibody.

Storage

Store the Napsin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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