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Home >> Antibodies >> Napsin A Antibody

Napsin A Antibody [clone NAPSA/1239] (V2996)

  Catalog No Formulation Size Price (USD)  
Image V2996-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V2996-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V2996SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
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V2996IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 499
IHC: Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with Napsin-A antibody (NAPSA/1239).
Western blot of 1) K562 and 2) HEK293 cell lysates using Napsin-A antibody (NAPSA/1239). Predicted molecular weight ~45 kDa.
SDS-PAGE Analysis of Purified, BSA-Free Napsin A Antibody (clone NAPSA/1239). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name NAPSA/1239
Purity Protein G affinity chromatography
UniProt O96009
Localization Cytoplasmic
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml for 60 min at RT
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Napsin A antibody is available for research use only.
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Description

Napsin-A is a protein that in humans is encoded by the NAPSA gene. The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [Wiki]

Application Notes

Optimal dilution of the Napsin A antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant fragment from the human protein (amino acids 189-299) was used as the immunogen for the Napsin A antibody.

Storage

Store the Napsin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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