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Home >> Antibodies >> Napsin A Antibody Cocktail

Napsin A Antibody Cocktail [clone NAPSA/1238 + NAPSA/1239] (V2997)

  Catalog No Formulation Size Price (USD)  
Image V2997-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V2997-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V2997SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
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V2997IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 499
IHC: Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with Napsin-A antibody (NAPSA/1238 + NAPSA/1239).
Western blot testing of A) K562, B) HEK293 and C) A549 lysate using Napsin A antibody. Predicted molecular weight ~45 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name NAPSA/1238 + NAPSA/1239
Purity Protein G affinity chromatography
UniProt O96009
Localization Cytoplasmic
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml for 60 min at RT
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Napsin A antibody cocktail is available for research use only.
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Description

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Application Notes

Optimal dilution of the Napsin A antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids 189-299 from the human protein were used as the immunogen for the mAbs in the Napsin A antibody cocktail.

Storage

Store the Napsin A antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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