NAPRT Antibody / NAD Salvage Pathway Enzyme Antibody (RQ8246)

NAPRT Antibody HeLa Cell IF. Immunofluorescence analysis of NAPRT Antibody / NAD Salvage Pathway Enzyme Antibody in HeLa cells. Cells were subjected to enzyme antigen retrieval, stained with NAPRT antibody at 5 ug/ml and detected with a Fluoro594-conjugated secondary antibody. Nuclei were counterstained with DAPI (blue). NAPRT staining is observed predominantly within the cytoplasm with punctate intracellular distribution, consistent with its role as a metabolic enzyme involved in NAD biosynthesis through the Preiss-Handler pathway. This NAPRT Antibody, a NAD Salvage Pathway Enzyme Antibody, is suitable for investigating NAPRT expression, subcellular localization and cellular NAD metabolism by immunofluorescence.

NAPRT Antibody Human, Rat and Mouse WB. Western blot analysis of NAPRT Antibody / NAD Salvage Pathway Enzyme Antibody detected endogenous NAPRT in cell and tissue lysates. Lane 1: human HeLa cells; Lane 2: rat liver; Lane 3: rat small intestine; Lane 4: mouse liver; Lane 5: mouse small intestine. A rabbit polyclonal NAPRT antibody was used at 0.5 ug/ml. A specific band is detected at approximately 58 kDa, consistent with the predicted molecular weight of NAPRT. This NAPRT Antibody, a NAD Salvage Pathway Enzyme Antibody, demonstrates cross-species reactivity and is suitable for studies of NAD biosynthesis, nicotinic acid metabolism and cellular bioenergetics.

NAPRT Antibody HepG2 Cell FACS. Flow cytometry analysis of NAPRT Antibody / NAD Salvage Pathway Enzyme Antibody in fixed and permeabilized HepG2 cells. Cells were stained with NAPRT antibody at 1 ug/10^6 cells, followed by a Fluoro488-conjugated secondary antibody. The blue histogram represents NAPRT staining, the green histogram is the rabbit IgG isotype control and the red histogram is the unstained control. A clear rightward shift of the NAPRT signal relative to both controls demonstrates specific intracellular detection of endogenous NAPRT. This NAPRT Antibody, a NAD Salvage Pathway Enzyme Antibody, is suitable for flow cytometric analysis of NAD biosynthesis and metabolic enzyme expression.