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Home >> Antibodies >> NANOG Antibody (N-Terminal Region)

NANOG Antibody (N-Terminal Region) (F44247)

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  Catalog No Formulation Size Price (USD)  
Image F44247-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
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F44247-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of NANOG antibody and K562 lysate; Predicted molecular weight: 35-45 kDa.
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Western blot analysis of NANOG antibody and K562 lysate; Predicted molecular weight: 35-45 kDa.
Western blot analysis of NANOG antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2). Predicted molecular weight: 35-45 kDa.
Fluorescent confocal image of SY5Y cells stained with NANOG antibody at 1:50. Immunoreactivity is localized mainly to the nuclei of the cells.
Fluorescent confocal image of HeLa cell stained with NANOG antibody at 1:25. Immunoreactivity is localized to the nucleus.
IHC analysis of FFPE human spleen tissue stained with NANOG antibody
NANOG antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Primate
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q9H9S0
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Flow Cytometry : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This NANOG antibody is available for research use only.
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Description

NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation. [UniProt]

Application Notes

Titration of the NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 15-49 from the human protein was used as the immunogen for this NANOG antibody.

Storage

Aliquot the NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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