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Home >> Antibodies >> N-Cadherin Antibody

N-Cadherin Antibody (R30391)

  Catalog No Formulation Size Price (USD)  
Image R30391 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of N-Cadherin antibody and Lane 1: MCF-7; 2: HeLa cell lysate. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).
IHC-P: N-Cadherin antibody testing of human ovary cancer tissue
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P19022
Localization Cell surface, cytoplasmic
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This N-Cadherin antibody is available for research use only.
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Description

NCAD is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). Human and mouse nucleotide sequences are 96% identical. The mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. The human Ncad gene contains 16 exons and its sequence is highly similar to both the mouse gene (including the large first and second introns) and other cadherin genes. Cadherins regulates dendritic spine morphogenesis.

Application Notes

The stated application concentrations are suggested starting points. Titration of the N-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 701-714 (CQCDSNGDCTDVDR) were used as the immunogen for this N-Cadherin antibody (100% homologous human, mouse and rat).

Storage

After reconstitution, the N-Cadherin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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