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Home >> Antibodies >> N-Cadherin Antibody / CDH2 / CD325

N-Cadherin Antibody / CDH2 / CD325 (R30391)

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  Catalog No Formulation Size Price (USD)  
Image R30391 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human colon cancer tissue with N-Cadherin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
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Immunofluorescent staining of FFPE human colon cancer tissue with N-Cadherin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE rat heart tissue with N-Cadherin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
IHC staining of FFPE human tonsil tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer tissue with N-Cadherin antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat heart tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain, 2) rat heart, 3) rat liver, 4) mouse heart, 5) mouse liver and 6) human SK-O-V3 cell lysate with N-Cadherin antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).
Flow cytometry testing of fixed human A549 cells with N-Cadherin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= N-Cadherin antibody.
IHC staining of FFPE human liver cancer tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human liver cancer tissue with N-Cadherin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human lung cancer tissue with N-Cadherin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human ovarian cancer tissue with N-Cadherin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE mouse testis tissue with N-Cadherin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE rat heart tissue with N-Cadherin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Western blot testing of 1) human 293T, 2) human PC-3, 3) human HeLa, 4) human A549, 5) rat brain, 6) rat C6, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with N-Cadherin antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P19022
Localization Cell surface, cytoplasmic
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Limitations This N-Cadherin antibody is available for research use only.
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Description

NCAD is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). Human and mouse nucleotide sequences are 96% identical. The mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. The human Ncad gene contains 16 exons and its sequence is highly similar to both the mouse gene (including the large first and second introns) and other cadherin genes. Cadherins regulates dendritic spine morphogenesis.

Application Notes

The stated application concentrations are suggested starting points. Titration of the N-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 701-714 (CQCDSNGDCTDVDR) were used as the immunogen for this N-Cadherin antibody (100% homologous human, mouse and rat).

Storage

After reconstitution, the N-Cadherin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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