N-Cadherin Antibody for IF / NCAD Antibody for Immunofluorescence (R30391)

N-Cadherin Antibody for IF Human Colon Cancer. Immunofluorescent staining of FFPE human colon cancer tissue with N-Cadherin Antibody (green) and DAPI nuclear stain (blue). Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes. Green fluorescence demonstrates N-Cadherin expression within the tumor tissue, with nuclei counterstained blue. This N-Cadherin Antibody for IF, also called NCAD Antibody for Immunofluorescence, supports visualization of CDH2 expression and localization in studies of cell-cell adhesion, epithelial-mesenchymal transition, and colorectal cancer biology.

N-Cadherin Antibody for IF Rat Heart Tissue. Immunofluorescent staining of FFPE rat heart tissue with N-Cadherin Antibody (green) and DAPI nuclear stain (blue). Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes. Green fluorescence demonstrates N-Cadherin expression within the cardiac tissue, with nuclei counterstained blue. This N-Cadherin Antibody for IF, also called NCAD Antibody for Immunofluorescence, supports visualization of CDH2 expression and localization in studies of cardiac cell adhesion, intercalated disc organization, and heart tissue biology.

IHC staining of FFPE human tonsil tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human ovarian cancer tissue with N-Cadherin antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

IHC staining of FFPE mouse heart tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat heart tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) rat brain, 2) rat heart, 3) rat liver, 4) mouse heart, 5) mouse liver and 6) human SK-O-V3 cell lysate with N-Cadherin antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).

Flow cytometry testing of fixed human A549 cells with N-Cadherin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= N-Cadherin antibody.

IHC staining of FFPE human liver cancer tissue with N-Cadherin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

N-Cadherin Antibody for IF Human Liver Cancer. Immunofluorescent staining of FFPE human liver cancer tissue with N-Cadherin Antibody (red) and DAPI nuclear stain (blue). Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes. Red fluorescence demonstrates N-Cadherin expression throughout the tumor tissue, with nuclei counterstained blue. This N-Cadherin Antibody for IF, also called NCAD Antibody for Immunofluorescence, supports visualization of CDH2 expression and localization in studies of cell-cell adhesion, epithelial-mesenchymal transition, and liver cancer biology.

N-Cadherin Antibody for IF Human Lung Cancer. Immunofluorescent staining of FFPE human lung cancer tissue with N-Cadherin Antibody (red) and DAPI nuclear stain (blue). Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes. Red fluorescence demonstrates N-Cadherin expression within the tumor tissue, with nuclei counterstained blue. This N-Cadherin Antibody for IF, also called NCAD Antibody for Immunofluorescence, supports visualization of CDH2 expression and localization in studies of cell-cell adhesion, epithelial-mesenchymal transition, and lung cancer biology.

N-Cadherin Antibody for IF Human Ovarian Cancer. Immunofluorescent staining of FFPE human ovarian cancer tissue with N-Cadherin Antibody (red) and DAPI nuclear stain (blue). Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes. Red fluorescence demonstrates N-Cadherin expression within the tumor tissue, with nuclei counterstained blue. This N-Cadherin Antibody for IF, also called NCAD Antibody for Immunofluorescence, supports visualization of CDH2 expression and localization in studies of cell-cell adhesion, epithelial-mesenchymal transition, and ovarian cancer biology.

N-Cadherin Antibody for IF Mouse Testis Tissue. Immunofluorescent staining of FFPE mouse testis tissue with N-Cadherin Antibody (red) and DAPI nuclear stain (blue). Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes. Red fluorescence demonstrates N-Cadherin expression within the testicular tissue, with nuclei counterstained blue. This N-Cadherin Antibody for IF, also called NCAD Antibody for Immunofluorescence, supports visualization of CDH2 expression and localization in studies of cell-cell adhesion, tissue organization, and reproductive biology.

N-Cadherin Antibody for IF Rat Cardiac Tissue. Immunofluorescent staining of FFPE rat heart tissue with N-Cadherin Antibody (red) and DAPI nuclear stain (blue). Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes. Red fluorescence demonstrates N-Cadherin expression within the cardiac tissue, with nuclei counterstained blue. This N-Cadherin Antibody for IF, also called NCAD Antibody for Immunofluorescence, supports visualization of CDH2 expression and localization in studies of cardiac cell adhesion, intercalated disc organization, and heart tissue biology.

Western blot testing of 1) human 293T, 2) human PC-3, 3) human HeLa, 4) human A549, 5) rat brain, 6) rat C6, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with N-Cadherin antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).