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Home >> Antibodies >> Myeloperoxidase Antibody / MPO

Myeloperoxidase Antibody / MPO (R30233)

  Catalog No Formulation Size Price (USD)  
Image R30233 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE mouse spleen tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lymph tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HL60, 2) rat thymus, 3) mouse spleen and 4) mouse thymus tissue with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).
Immunofluorescent staining of FFPE human spleen tissue with Myeloperoxidase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human appendicitis tissue with Myeloperoxidase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Flow cytometry testing of human HL60 cells with Myeloperoxidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Myeloperoxidase antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P05164
Localization Nuclear and cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Limitations This Myeloperoxidase antibody is available for research use only.
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Description

Myeloperoxidase is a mammalian phagocyte hemoprotein though to primarily mediate host defense reactions. It is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms.

Application Notes

Titration of the pAb may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of the human protein (KNNIFMSNSYPRDFV) was used as the immunogen for this Myeloperoxidase antibody.

Storage

The lyophilized Myeloperoxidase antibody can be stored at 4oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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